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mito dsred2  (TaKaRa)


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    Structured Review

    TaKaRa mito dsred2
    Mito Dsred2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mito+dsred2/pDsRed2-Mito+Vector/pmc12810648-129-4-5
    Average 95 stars, based on 402 article reviews
    mito dsred2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Transfection:

    Article Title: Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells
    Article Snippet: The cells were then transfected with 2 μg DNA constructs in serum-free RPMI media (5 h, 37 °C, 5% CO 2 ) using Lipofectamine 2000 (Life Technologies, Carlsbad, CA). .. Transfected constructs included CgA-GFP ( Taupenot et al., 2002 ), calreticulin-EYFP (pEYFP-ER plasmid; Clontech Laboratories, Inc., Mountain View, CA) and Mito-dsRed2 (pDsRed2-Mito plasmid; Clontech Laboratories, Inc.). ..

    Construct:

    Article Title: Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells
    Article Snippet: The cells were then transfected with 2 μg DNA constructs in serum-free RPMI media (5 h, 37 °C, 5% CO 2 ) using Lipofectamine 2000 (Life Technologies, Carlsbad, CA). .. Transfected constructs included CgA-GFP ( Taupenot et al., 2002 ), calreticulin-EYFP (pEYFP-ER plasmid; Clontech Laboratories, Inc., Mountain View, CA) and Mito-dsRed2 (pDsRed2-Mito plasmid; Clontech Laboratories, Inc.). ..

    Plasmid Preparation:

    Article Title: Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells
    Article Snippet: The cells were then transfected with 2 μg DNA constructs in serum-free RPMI media (5 h, 37 °C, 5% CO 2 ) using Lipofectamine 2000 (Life Technologies, Carlsbad, CA). .. Transfected constructs included CgA-GFP ( Taupenot et al., 2002 ), calreticulin-EYFP (pEYFP-ER plasmid; Clontech Laboratories, Inc., Mountain View, CA) and Mito-dsRed2 (pDsRed2-Mito plasmid; Clontech Laboratories, Inc.). ..

    Article Title: Anxiety-associated behaviors following ablation of Miro1 from cortical excitatory neurons.
    Article Snippet: The ANY-MAZE software (Stoelting Co.) was used to track the path of 230 the mice in the box and measure the time spent in the narrow and wide sections of the box. .. 231 Plasmid Construction 232 Separate fragments of EGFP, mito-DsRed2 (TaKaRa, Cat #:632421) and P2A derived from 233 porcine teschovirus-1 2A (Kim et al, 2011) were amplified by PCR. .. The fragments were 234 subcloned into pCAG (Addgene) by Geneart (Thermofisher).

    Article Title: Defective mitochondrial-lysosomal axis promotes extracellular vesicles release of mitochondrial components in Huntington’s Disease
    Article Snippet: .. The 86 bp insert of Mito-DsRed2 (Clontech) was subcloned at the NheI and BsrGI sites of the pCL6EGwo vector using standard recombinant DNA technology (pCL6-Mito-DsRed2). ..

    Article Title: Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington's disease
    Article Snippet: .. The 86 bp insert of Mito-DsRed2 (Clontech) was subcloned at the NheI and BsrGI sites of the pCL6EGwo vector using standard recombinant DNA technology (pCL6-Mito-DsRed2). ..

    Derivative Assay:

    Article Title: Anxiety-Associated Behaviors Following Ablation of Miro1 from Cortical Excitatory Neurons
    Article Snippet: The ANY-maze software (Stoelting) was used to track the path of the mice in the box and measure the time spent in the narrow and wide sections of the box. .. Separate fragments of EGFP, mito-DsRed2 (TaKaRa, catalog #632421), and P2A derived from porcine teschovirus-1 2A were amplified by PCR. .. The fragments were subcloned into pCAG (Addgene) by Geneart (Thermo Fisher Scientific).

    Article Title: Anxiety-associated behaviors following ablation of Miro1 from cortical excitatory neurons.
    Article Snippet: The ANY-MAZE software (Stoelting Co.) was used to track the path of 230 the mice in the box and measure the time spent in the narrow and wide sections of the box. .. 231 Plasmid Construction 232 Separate fragments of EGFP, mito-DsRed2 (TaKaRa, Cat #:632421) and P2A derived from 233 porcine teschovirus-1 2A (Kim et al, 2011) were amplified by PCR. .. The fragments were 234 subcloned into pCAG (Addgene) by Geneart (Thermofisher).

    Article Title: Anxiety-associated behaviors following ablation of Miro1 from cortical excitatory neurons
    Article Snippet: The ANY-MAZE software (Stoelting Co.) was used to track the path of the mice in the box and measure the time spent in the narrow and wide sections of the box. .. Separate fragments of EGFP, mito-DsRed2 (TaKaRa, Cat #:632421) and P2A derived from porcine teschovirus-1 2A (Kim et al, 2011) were amplified by PCR. .. The fragments were subcloned into pCAG (Addgene) by Geneart (Thermofisher).

    Amplification:

    Article Title: Anxiety-Associated Behaviors Following Ablation of Miro1 from Cortical Excitatory Neurons
    Article Snippet: The ANY-maze software (Stoelting) was used to track the path of the mice in the box and measure the time spent in the narrow and wide sections of the box. .. Separate fragments of EGFP, mito-DsRed2 (TaKaRa, catalog #632421), and P2A derived from porcine teschovirus-1 2A were amplified by PCR. .. The fragments were subcloned into pCAG (Addgene) by Geneart (Thermo Fisher Scientific).

    Article Title: Anxiety-associated behaviors following ablation of Miro1 from cortical excitatory neurons.
    Article Snippet: The ANY-MAZE software (Stoelting Co.) was used to track the path of 230 the mice in the box and measure the time spent in the narrow and wide sections of the box. .. 231 Plasmid Construction 232 Separate fragments of EGFP, mito-DsRed2 (TaKaRa, Cat #:632421) and P2A derived from 233 porcine teschovirus-1 2A (Kim et al, 2011) were amplified by PCR. .. The fragments were 234 subcloned into pCAG (Addgene) by Geneart (Thermofisher).

    Article Title: Anxiety-associated behaviors following ablation of Miro1 from cortical excitatory neurons
    Article Snippet: The ANY-MAZE software (Stoelting Co.) was used to track the path of the mice in the box and measure the time spent in the narrow and wide sections of the box. .. Separate fragments of EGFP, mito-DsRed2 (TaKaRa, Cat #:632421) and P2A derived from porcine teschovirus-1 2A (Kim et al, 2011) were amplified by PCR. .. The fragments were subcloned into pCAG (Addgene) by Geneart (Thermofisher).

    Polymerase Chain Reaction:

    Article Title: Anxiety-Associated Behaviors Following Ablation of Miro1 from Cortical Excitatory Neurons
    Article Snippet: The ANY-maze software (Stoelting) was used to track the path of the mice in the box and measure the time spent in the narrow and wide sections of the box. .. Separate fragments of EGFP, mito-DsRed2 (TaKaRa, catalog #632421), and P2A derived from porcine teschovirus-1 2A were amplified by PCR. .. The fragments were subcloned into pCAG (Addgene) by Geneart (Thermo Fisher Scientific).

    Article Title: Anxiety-associated behaviors following ablation of Miro1 from cortical excitatory neurons.
    Article Snippet: The ANY-MAZE software (Stoelting Co.) was used to track the path of 230 the mice in the box and measure the time spent in the narrow and wide sections of the box. .. 231 Plasmid Construction 232 Separate fragments of EGFP, mito-DsRed2 (TaKaRa, Cat #:632421) and P2A derived from 233 porcine teschovirus-1 2A (Kim et al, 2011) were amplified by PCR. .. The fragments were 234 subcloned into pCAG (Addgene) by Geneart (Thermofisher).

    Article Title: Anxiety-associated behaviors following ablation of Miro1 from cortical excitatory neurons
    Article Snippet: The ANY-MAZE software (Stoelting Co.) was used to track the path of the mice in the box and measure the time spent in the narrow and wide sections of the box. .. Separate fragments of EGFP, mito-DsRed2 (TaKaRa, Cat #:632421) and P2A derived from porcine teschovirus-1 2A (Kim et al, 2011) were amplified by PCR. .. The fragments were subcloned into pCAG (Addgene) by Geneart (Thermofisher).

    Recombinant:

    Article Title: Defective mitochondrial-lysosomal axis promotes extracellular vesicles release of mitochondrial components in Huntington’s Disease
    Article Snippet: .. The 86 bp insert of Mito-DsRed2 (Clontech) was subcloned at the NheI and BsrGI sites of the pCL6EGwo vector using standard recombinant DNA technology (pCL6-Mito-DsRed2). ..

    Article Title: Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington's disease
    Article Snippet: .. The 86 bp insert of Mito-DsRed2 (Clontech) was subcloned at the NheI and BsrGI sites of the pCL6EGwo vector using standard recombinant DNA technology (pCL6-Mito-DsRed2). ..



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    Image Search Results


    Image acquisition immediately after astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta) are co-cultured with neurons expressing mito-mTagBFP2 (blue).

    Journal: Cell Reports Methods

    Article Title: MitoTracker transfers from astrocytes to neurons independently of mitochondria

    doi: 10.1016/j.crmeth.2026.101338

    Figure Lengend Snippet: Image acquisition immediately after astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta) are co-cultured with neurons expressing mito-mTagBFP2 (blue).

    Article Snippet: Sequences can be found in Methods S1. pDsRed2-Mito-7 was a gift from Michael Davidson (Addgene plasmid #55838; http://n2t.net/addgene:55838; RRID:Addgene_55838). pZac2.1-GfaABC1D-TurboID(full)-HA-GPI was a gift from Scott Soderling (Addgene plasmid #166055; http://n2t.net/addgene:166055; RRID:Addgene_166055).

    Techniques:

    Neurons expressing mTagBFP2 (blue) are incubated with ACM from astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta).

    Journal: Cell Reports Methods

    Article Title: MitoTracker transfers from astrocytes to neurons independently of mitochondria

    doi: 10.1016/j.crmeth.2026.101338

    Figure Lengend Snippet: Neurons expressing mTagBFP2 (blue) are incubated with ACM from astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta).

    Article Snippet: Sequences can be found in Methods S1. pDsRed2-Mito-7 was a gift from Michael Davidson (Addgene plasmid #55838; http://n2t.net/addgene:55838; RRID:Addgene_55838). pZac2.1-GfaABC1D-TurboID(full)-HA-GPI was a gift from Scott Soderling (Addgene plasmid #166055; http://n2t.net/addgene:166055; RRID:Addgene_166055).

    Techniques: